When the peptide is conjugated to ligands of interest, we demonstrate the photo-activated cellular uptake of a wide range of cargoes, including small fluorophores, proteins, inorganic ( e.g., quantum dots and gold nanostars) and organic nanomaterials ( e.g., polymeric particles), and liposomes.
To achieve this, we designed a novel photo-caged peptide which undergoes a structural transition from an antifouling ligand to a cell-penetrating peptide upon photo-irradiation. Here we report new photo-activatable cell–material interfacing systems that trigger cellular uptake of various cargoes and cell adhesion towards surfaces. Furthermore, it was observed that with increase in norbornene content, the grafting efficiency decreases resulting in poor surface modification.Spatio-temporally tailoring cell–material interactions is essential for developing smart delivery systems and intelligent biointerfaces. It was observed that in hot embossing, higher norbornene content contributed to good replication fidelity at identical experimental conditions. The modified surface was evaluated using Fourier transform infrared spectroscopy (FTIR spectroscopy) and water contact angle measurement. Replication fidelity which includes the surface morphology and cross-section profiles of the microchannel were characterized using SEM and Confocal microscope respectively. We report in this paper, a systematic study of replication fidelity and surface modification on COC polymer with varying norbornene content from 65 to 82 wt%. For copolymers such as cyclic olefin copolymer (COC) which comprises ethylene and norbornene units, such properties depend on their relative ethylene and norbornene content. During hot embossing, the replication fidelity on polymer substrate not only depends on the processing parameters such as temperature, pressure and time but also on their chemical structure which affects their thermo-dependent viscoelastic properties. The inner fluffy meltblown layer of SMS nonwovens favors the grafting as compared with the SB nonwovens.Ībstract: The fabrication of polymer based microfluidic devices using the hot embossing technique and their surface modification for easy fluid flow through the devices has been a growing field of research. The tight texture structure and the well-stretched monofilament surfaces of the MPF fabrics retard the grafting to a great extent. The results showed that, the grafting degrees of the two monomers increase and then tend constant with irradiation time, while those increase with the increase of the monomer concentrations for all the fabrics. The infrared spectra and the scanning electron microscopy images verified the successful grafting copolymerization of the two monomers, glycidyl methacrylate (GMA) and Hydroxyethyl methacrylate (HEMA), onto the three fabrics, the spunbonded polypropylene nonwovens (SB), the spunlaid-meltblown-spunlaid polypropylene nonwovens (SMS), and the polypropylene monofilament plain fabrics (MPF). Abstract: The effect of the polypropylene fabric morphology on the grafting copolymerization of methacrylics in the aqueous solution under ultraviolet irradiation was investigated.
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